Isolation of microorganisms capable of degrading trinitrotoluene (TNT) from contaminated soil and water
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https://doi.org/10.54939/1859-1043.j.mst.78.2022.132-139Keywords:
Microorganism; Isolation; TNT.Abstract
TNT (2,4,6 trinitrotoluene) is an important explosive with a highly chemical durability, but it is also a highly toxic compound. TNT can be degraded by different pathways, one of which is biodegradation. In this study, we isolated microorganisms from TNT contaminated soil and water and surveyed their TNT’s disintegration capability. During degradation, treatment of TNT after 5 days of incubation was followed by a spectrophotometer and analyzed by high-performance liquid chromatography (HPLC). According to the results of the analysis that have done, the TN1 and Z121 strains are able to degrade TNT at an initial concentration of 50 mg/L with 50,24% and 65,28%, respectively. These isolates were identified through PCR reaction with 16 S rRNA sequence analysis method through. According to the sequence of 16S rRNA, TN1 strain belonged to Pseudomonas genus, Z121 strain belonged to Klebsiella genus with the GenBank code is MZ165355.1 and MZ165349.1, respectively.
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